In a Heartbeat

Two hearts, said Keats, can beat as one; but a study led by Weizmann Institute scientists in collaboration with researchers from the University of Pennsylvania shows that sometimes a single heart muscle cell can beat as more than two dozen. The findings, reported recently in Nature Communications, provide an extremely detailed glimpse into the mechanisms behind normal and irregular heart muscle cell contractions. The study may help define the limitations of existing therapies for abnormal heartbeat and, in the future, suggest ways of designing new ones.

Each heart muscle cell consists of numerous parallel filaments comprising repeated subunits. When the heart beats, each individual filament contracts to produce muscle cell contractions.

Optimally, all the filaments should contract in a synchronized manner, thus ensuring the greatest amplitude of contraction for each muscle cell, and ultimately, the strongest and most effective beating of the entire heart. However, a new theoretical model proposed and analyzed by Prof. Samuel Safran and postdoctoral fellow Dr. Kinjal Dasbiswas of the Weizmann Institute’s Materials and Interfaces Department suggests that the filaments contract together only when their subunits, and subunit boundaries, are aligned with one another. Since such alignment usually only happens among a limited number of neighboring filaments, aligned filaments contract together as a bundle, but each such bundle contracts out of phase with others. Therefore, a heart cell does not necessarily beat as a single uniform entity; rather, the number of different beating entities in the cell depends on the bundle number, which may reach more than two dozen.

The theory, which uses the methods of statistical physics, further predicted that the alignment of the filaments in the heart muscle cell depends on the cell’s physical environment; more specifically on the elasticity of the supporting structure called the extracellular matrix. The alignment is best when this structure is not too soft and not too rigid. The prediction took into consideration various forces operating on the microscale, particularly mechanical forces that are exerted on each filament subunit by neighboring filaments via the extracellular matrix.  

 

 

 

 

 

 

 

 

 

 

 

 

 

By assuming that only  structurally aligned filaments beat together,  the Weizmann theorists were able to quantitatively explain experimental findings by their collaborators from the University of Pennsylvania, Prof. Dennis Discher and Dr. Stephanie Majkut. In the experiments, these scientists had placed chick embryonic heart cells on support surfaces of varying stiffness and found that two strikingly different properties – the structural alignment of the filaments and the beating strength of the cell – depended on the rigidity of the supporting surface.