When Age Matters

English
A partial human skull unearthed in 2008 in northern Israel may hold some clues as to when and where humans and Neanderthals might have interbred. The key to addressing this, as well as other important issues, is precisely determining the age of the skull. A combination of dating methods, one of them performed by Dr. Elisabetta Boaretto, head of the Weizmann Institute’s D-REAMS (DANGOOR Research Accelerator Mass Spectrometry) laboratory, has made it possible to define the period of time that the cave was occupied and thus the skull’s age. The combined dating provides evidence that Homo sapiens and Homo neanderthalensis could have lived side by side in the area.
 
Manot skull
 
The Manot Cave, a natural limestone formation, had been sealed for some 15,000 years. It was discovered by a bulldozer clearing the land for development, and the first to find the partial skull, which was sitting on a ledge, were spelunkers exploring the newly-opened cave. Five excavation seasons uncovered a rich deposit, with stone tools and stratified occupation levels covering a period of time from at least 55,000 to 27,000 years ago.
 
 
Dating the skull presented a number of difficulties. “Because it was already removed from the layer where it was presumably deposited,” says Dr. Elisabetta Boaretto, “we had to look for clues to tell us where and when it belonged in the setting of the archaeological record in the cave.”

The age of the skull was first determined to be 54.7 thousand years old by a technique known as the uranium-thorium method, which was applied to the thin mineral deposit on the skull. But the estimated possible error in that type of method is plus or minus 5.5 thousand years. To obtain independent confirmation of the date, a different, independent type of dating was required, e.g., radiocarbon dating.

To narrow down the possible range of the skull’s age and determine when the skull’s owner had lived in the cave, the archaeological team led by Prof. Israel Hershkovitz of Tel Aviv University, Dr. Ofer Marder of Ben Gurion University and Dr. Omry Barzilai of the Israel Antiquities Authority turned to Dr. Boaretto. She and her team participated in the excavation of the cave and applied radiocarbon dating to carefully selected charcoal remains, so that the whole cave, and thus the timing of human occupation, was mapped. The agreement between the two methods – carbon and uranium-thorium – provided the necessary support for the “correction” in the original uranium-thorium dating of the skull, which then helped fix the true age of the skull at around 55,000 years.

The date and shape of the Manot Cave skull provides some intriguing evidence that humans and Neanderthals might have interbred sometime during the human trek out of Africa, most likely as the former passed through the Middle East before spreading out north and east. The 55,000-year-old partial skull is the first evidence of a human residing in the region at the same time as Neanderthals, whose remains have been found at several nearby sites. Archaeologists are now searching for more evidence of ancient human habitation in the cave. If, indeed, the mixing between humans and Neanderthals took place in this area, it would suggest that the owner of the skull and his kin may have been the ancestors of all modern non-Africans.  



 
 
Manot skull
Scientific Archaeology
English
Yes

300,000-Year-Old Hearth Found

English

Humans, by most estimates, discovered fire over a million years ago. But when did they really begin to control fire and use it for their daily needs? That question – one which is central to the subject of the rise of human culture – is still hotly debated. A team of Israeli scientists recently discovered in the Qesem Cave, an archaeological site near present-day Rosh Ha’ayin, the earliest evidence – dating to around 300,000 years ago – of unequivocal repeated fire building over a continuous period. These findings not only help answer the question, they hint that those prehistoric humans already had a highly advanced social structure and intellectual capacity.


Excavations in Qesem Cave have been ongoing since 2000. The team is headed by Profs. Avi Gopher and Ran Barkai of Tel Aviv University. Dr. Ruth Shahack-Gross of the Kimmel Center for Archeological Science at the Weizmann Institute has been involved in this archaeological research since excavations began, and she collects samples on-site for later detailed analysis in the lab. Shahack-Gross, whose expertise is in the identification of archaeological materials, identified a thick deposit of wood ash in the center of the cave. Using infrared spectroscopy, she and her colleagues were able to determine that mixed in with the ash were bits of bone and soil that had been heated to very high temperatures. This was conclusive proof that the area had been the site of a large hearth.
 
Scan of a sediment “slice” from the hearth area of the cave showing burnt bone and rock fragments within the gray ash residue
 
Next, Shahack-Gross tested the micro-morphology of the ash. To do this, she extracted a cubic chunk of sediment from the hearth and hardened it in the lab. Then she sliced it into extremely thin slices – so thin they could be placed under a microscope to observe the exact composition of the materials in the deposit and reveal how they were formed. With this method, she was able to distinguish a great many micro-strata in the ash – evidence for a hearth that was used repeatedly over time. These findings were published in the Journal of Archaeological Science.

Around the hearth area, as well as inside it, the archaeologists found large numbers of flint tools that were clearly used for cutting meat. In contrast, the flint tools found just a few meters away had a different shape, designed for other activities. Also in and around the area were large numbers of burnt animal bones – further evidence for repeated fire use for cooking meat. Shahack-Gross and her colleagues have shown that this organization of various “household” activities into different parts of the cave points to an organization of space – and a thus kind of social order – that is typical of modern humans. This suggests that the cave was a sort of base camp that prehistoric humans returned to again and again. “These findings help us to fix an important turning point in the development of human culture – that in which humans first began to regularly use fire both for cooking meat and as a focal point – a sort of campfire – for social gatherings,” she says. “They also tell us something about the impressive levels of social and cognitive development of humans living some 300,000 years ago.” The researchers think that these findings, along with others, are signs of substantial changes in human behavior and biology that commenced with the appearance in the region of new forms of culture – and indeed a new human species – about 400,000 years ago.
        
Upper left: Infrared spectrum of the grey sediments, right, showing that the dominant material is calcite, the mineral of which the wood ash is composed. Lower left: Photograph of the cave during excavation; arrow pointing to the hearth. Upper right: micro-morphological image of the grey sediment showing dark grey particles and patches corresponding to the remains of wood ash. Lower right: Scan of a micro-morphological, thin section showing the layered burnt bones (yellow, brown and black fragments), intermixed with grey sediments
 
 

 

 

 
 
Photograph of the cave during excavation; arrow pointing to the hearth.
Scientific Archaeology
English
Yes

Oldest Use of Flowers in Grave Lining

English

 

When did people first begin to express their feelings with flowers? It turns out that in prehistoric times, Mount Carmel residents in what today is northern Israel buried their dead on a literal bed of fragrant wild flowers, such as Judean sage, as well as blooming plants of the mint and figwort families. Assuming they had the same positive associations with flowers that we do today, these ancient humans must have sought to ensure for the deceased a pleasant passage from the world of the living.

The discovery is the oldest known use of flowers in grave lining. According to radiocarbon dating performed by Dr. Elisabetta Boaretto at the Weizmann Institute of Science, the graves are 11,700 to 13,700 years old. Boaretto was part of an international team, headed by archaeologist Prof. Dani Nadel of the University of Haifa, who performed excavations in the Raqefet Cave overlooking the Mediterranean Sea. It had been inhabited by the Natufians, prehistoric hunter-gatherers who were widespread in the Near East. The findings were reported recently in the Proceedings of the National Academy of Sciences, USA.
 
(left) Field photograph of two skeletons (adult on left, adolescent on right) during excavation. Photo: E. Gerstein, Haifa University (right) Reconstruction of the double burial at the time of inhumation. The bright veneer inside the grave on the right is partially covered by green plants
 

 

 
Boaretto and her group at the Weizmann Institute are currently exploring additional fascinating questions from the distant past, among them: When exactly did modern humans leave Africa, ultimately replacing the Neanderthals in the Near East and Europe?  Is it possible to use the tiny mineral remains of plants for dating ancient sites? Did the collapse of empires in the Early Bronze Age occur earlier than previously thought?

These questions are being addressed with the help of advanced equipment – the first of its kind in the entire Middle East – set up recently in the building that previously housed a particle accelerator. The newly-installed technology – the Dangoor-Research Accelerator Mass Spectrometer, or D-REAMS – is used to determine the age of archaeological samples by measuring the concentration of the radioactive carbon, or 14C. The method is based on determining the ratio between 14C and the stable carbon atoms, 12C and 13C: since 14C decays over time while the amount of stable carbon remains constant, the fewer radioactive atoms are found in a sample compared to the stable ones, the older the sample.

In the past, radiocarbon dating required relatively large amounts of material, at least several grams, because it relied on measuring 14C indirectly, by observing its decay. In contrast, an accelerator mass spectrometer like D-REAMS, which accelerates atoms to high energies before analysis, directly counts the radiocarbon atoms. Its precision is remarkable, considering that for every radioactive 14C there are anywhere between a trillion to a quadrillion stable carbon atoms.  

As a result, dating can be performed on a sample as small as a few milligrams. “A 5-gram sample, the equivalent of a small sugar packet, could yield about 5,000 measurements,” says Boaretto, who heads the D-REAMS laboratory.  “It is even possible to date a single seed.”

D-REAMS can shed new light on the distant past thanks to the high precision of its dating.  It can, for example, help determine when our ancestors, early Homo sapiens, migrated out of Africa. One of their first stops in the Near East was the site of Boker Tachtit, located in a sun-scorched ravine in Israel’s southern Negev desert. Radiocarbon dating of the material from an earlier excavation of Boker Tachtit in the 1970s suggested that the site was occupied about 47,000 years ago. But the dating method at the time was imprecise, so the question has remained open: When exactly was Boqer Tachtit inhabited?
 
Reconstructing ancient lifestyles
 
To answer this question, Boaretto and archaeologist Dr. Omry Barzilai from the Israel Antiquities Authority have obtained a license to re-excavate Boqer Tachtit, a project scheduled to begin in October 2013. Setting up an on-site laboratory, an international team of scientists including colleagues from the Max Planck Society in Leipzig, will collect charcoal remains from the fireplaces left by the early settlers, as well as sediments, bone fragments and other materials that can help them reconstruct the lifestyle of ancient humans, in addition to accurately dating the time of occupation. Back at Weizmann, the samples for dating will be analyzed in the D-REAMS laboratory.

The analysis can enable researchers, for instance, to clarify the relations of those early Homo sapiens with the Neanderthals who are thought to have also inhabited the area. To know if Homo sapiens interacted with these close relatives of theirs, it’s important to establish whether they actually inhabited the same regions at the same times. The study can also help researchers assess the pace of changes that took place in technology and lifestyle of the early humans.

The ability to analyze minute amounts of material is particularly important for such prehistoric sites as Boker Tachtit, where most of the analysis is performed at the microscopic level. With the help of D-REAMS, it is possible to build new dating methodology and tailor the sampling of the material in the field to the chronological question being asked.

The small sample size might make it possible to develop an entirely new approach to the dating of archaeological sites – a possibility currently under investigation in the D-REAMS laboratory. The approach would rely on the minute amounts of organic material trapped inside phytoliths –  tiny silica particles produced by many plants. While organic matter from plants disintegrates quickly, the durable inorganic phytoliths remain intact over millennia, and they are abundant in almost all archaeological sites. Even with the new technology, analyzing phytoliths for the purposes of radiocarbon dating poses an enormous challenge because they contain only about one-tenth of a percent of organic matter.
 
Surprising discovery
 
The high precision of D-REAMS analysis might be crucial in yet another project, which concerns the chronology of a relatively recent period: the Early Bronze Age, thought to have ended in the Near East some 4300 years ago. This is the period during which writing was developed in Mesopotamia and the first city states were created in the region. The end of the Early Bronze Age is defined by the abandonment of these cities.

Archaeologists have proposed that the city states had been abandoned due to the extreme drought known to have occurred in the region at the time. But in a recent study, Boaretto and her student Johanna Regev made a surprising discovery: At least in ancient Israel, the cities were abandoned – and, consequently, the Early Bronze ended – 200 years earlier than previously believed. This finding means that the theory linking the abandonment to dire climatic conditions no longer holds, so a new explanation is in order.

The revision might also have far-reaching implications for the study of the Bronze Age in the neighboring empires of Egypt and Mesopotamia, which were linked to ancient Israel by commerce and other ties. With the help of D-REAMS, scientists plan to explore the chronology of the Early Bronze Age further using material from different sites.
 
Dr. Elisabetta Boaretto's research is supported by the Exilarch's Foundation
 
(left) Field photograph of two skeletons (adult on left, adolescent on right) during excavation. Photo: E. Gerstein, Haifa University (right) Reconstruction of the double burial at the time of inhumation. The bright veneer inside the grave on the right is partially covered by green plants
Scientific Archaeology
English
Yes

New Center Looks to the Past

English

 

 Dr. Elisabetta Boaretto and Prof. Stephen Weiner

When did modern humans arrive in Europe and Asia? At what rate have cultural changes spread from one region to another throughout history? How did Neanderthal teeth and bones differ from ours? These and many other topics will be investigated at the new Max Planck – Weizmann Institute of Science Center in the Field of Integrative Archaeology and Anthropology.

The Center’s directors are Prof. Stephen Weiner of the Weizmann Institute and Prof. Jean-Jacques Hublin of the Max Planck Institute for Evolutionary Anthropology. Activities in the Center will be undertaken by two groups of scientists, in Israel and in Germany, each consisting of approximately 10 scientists and students. In addition to performing their own research, the groups will engage in collaborative activities between the Weizmann Institute and the Max Planck Institute.

Two separate tracks will focus on “The Timing of Cultural Change” and “Physical Anthropology through Bone and Tooth Structure-Function Studies,” and both will make use of scientific and technological advances developed in Weizmann Institute labs.

Among these advances is the planned installation of new accelerator mass spectrometry (AMS) equipment in the Institute’s Physics Faculty. The new radiocarbon lab, directed by Dr. Elisabetta Boaretto, is expected to have a major impact on archaeology research both locally and internationally. Because the AMS – which will be the only machine of its kind in the entire Middle East – is capable of detecting C-14 in extremely minute concentrations, it will be possible to perform the dating very fast and on minute quantities of material, such as a single lentil or grain of wheat, or a small trace of collagen in bones. This is an essential feature, because over thousands of years, organic matter on which radiocarbon dating is based tends to diminish and disappear. The new AMS lab, made possible by a gift from Dr. Naim Dangoor CBE of London, founder and head of the Exilarch's Foundation, is expected to be the first radiocarbon-dating laboratory in the world dedicated to research rather than to providing a service.
 
Prof. Stephen Weiner's research is supported by the Exilarch's Foundation; the Maurice and Vivienne Wohl Charitable Foundation; the estate of Hilda Jacoby-Schaerf; the Helen and Martin Kimmel Center for Archaeological Science, which he heads; the European Research Council; and the J&R Center for Scientific Research. Prof. Weiner is the incumbent of the Dr. Walter and Dr. Trude Borchardt Professorial Chair in Structural Biology.
 

 

 
 Dr. Elisabetta Boaretto and Prof. Stephen Weiner
Scientific Archaeology
English
Yes

Weizmann Institute and Max Planck Society Establish a Joint Center for Archaeology and Anthropology

English
When did modern humans arrive in Europe and Asia? At what rate have cultural changes spread from one region to another throughout history? How did Neanderthal teeth and bones differ from ours? These are examples of topics to be investigated at the new Max Planck – Weizmann Institute of Science Center in the Field of Integrative Archaeology and Anthropology.
 
The agreement for the establishment of the Center is being signed today at the Weizmann Institute of Science in Rehovot – for the Max Planck Society for the Advancement of Science, by Prof. Peter Gruss, President; and for the Weizmann Institute, by Prof. Daniel Zajfman, President. Serving as the Center’s Directors will be Prof. Stephen Weiner of the Weizmann Institute and Prof. Jean-Jacques Hublin of the Max Planck Institute for Evolutionary Anthropology.
 
The creation of the Center marks more than five decades of collaboration between the Max Planck Society and the Weizmann Institute. This collaboration, which originated in the late 1950s, led to the historic 1964 agreement whereby the Minerva Foundation for Research, a subsidiary of the Max Planck Society, channeled funds provided by the German government to Weizmann Institute research projects, thus fostering a wide range of scientific exchanges between the Institute and the Max Planck Society and other German Universities. These ties helped lay the foundation not only for German-Israeli scientific cooperation, but also for the establishment of diplomatic relations between the two countries one year later.
 
Apart from promoting the ties between the Max Planck Society and the Weizmann Institute, the new Center might serve as the basis for expanding scientific ties between Israel and its neighbors. ‘It would be natural to collaborate with our neighboring countries because we share roughly the same archaeological record,’ said Weizmann Institute’s Prof. Weiner. ‘Just as happened in relations with Germany, now too scientific collaboration could have a broader impact, helping to promote peaceful ties in the Middle East.’
 
Activities in the Center will be performed by two new groups of scientists in Israel and Germany, each numbering approximately 10 scientists and students. In addition to performing their own research, the groups will engage in collaborative activities between the Weizmann Institute and the Max Planck Institute.
 
The group at the Weizmann Institute in Rehovot will mainly follow the research track entitled ‘The Timing of Cultural Change.’ Its goal: to shed new light on such fascinating aspects of human history as the spread of ideas, the changes in lifestyles, the different rates of development in various parts of the world and the migration of people from one geographical area to another. Traditionally, these questions have been explored by relative dating – that is, comparing changes in tools or pottery in different regions. However, absolute dating – determining the actual age of objects and strata – is needed in order to establish when a particular change occurred and how fast it spread throughout the region. To document the distribution of cultural changes in the last 50,000 years, the scientists will conduct much of the work in the field, performing a scientific analysis of findings at the archaeological site itself, to be followed up by laboratory studies. They will use high-resolution radiocarbon dating, which makes it possible to date specimens with a precision of 20 to 40 years, taking advantage of such advanced techniques as accelerator mass spectrometry (AMS—see below) analysis of radiocarbon content.
 
The group at the Max Planck Institute for Evolutionary Anthropology in Leipzig will mainly conduct research along the track entitled ‘Physical Anthropology through Bone and Tooth Structure-Function Studies.’ Scientists in this group will investigate issues in recent human evolution, particularly those relating to the co-occurrence of Neanderthal and early modern human populations in the Levantine region, at the crossroads between Africa and Eurasia. The study of fossil remains of these two populations has been traditionally based on the shapes of bones and teeth, examined more recently with the help of 3D computer reconstructions. Scientists in this track will make use of high-resolution computer tomography both at the Max Planck Institute in Leipzig and at the Weizmann Institute, a technology that makes it possible to perform such reconstructions down to the level of micron-sized details. The scientists will examine the relationship between structure and function in bones and teeth, which is essential for understanding evolutionary changes. Since this relationship is difficult to establish using fossils alone, the focus of the studies in the new Center will be on modern bones and teeth.
 


Particle Accelerator for the Study of the Past

 
The new AMS equipment is expected to have a major impact on archaeology research both locally and internationally, as the only machine of its kind in the entire Middle East. Designed especially for conducting mainly archaeological research, it will be installed at the Weizmann Institute in a designated laboratory in the Physics Faculty, in the end of 2012. Archaeological dating used to rely on counters tracking the decay of the radioactive carbon isotope called C-14, a time-consuming process that requires large quantities of material. In contrast, AMS performs direct measurements of C-14, by accelerating the carbon atoms to a high speed and separating out the C-14 even when it is present at the minute concentrations of one in a quadrillion (1 followed by 15 zeroes) carbon atoms. This approach makes it possible to perform the dating very fast and on minute quantities of material, such as a single lentil, a grain of wheat or a small trace of collagen in bones – an essential feature, since over thousands of years, organic matter on which radiocarbon dating is based tends to disappear.
 
The gift that enabled the purchase of the accelerator mass spectrometer (AMS) came from Dr. Naim Dangoor CBE of London, founder and head of the Exilarch's Foundation. The Exilarch’s Foundation supports community and educational causes. The new AMS lab will be named DREAMS, for Dangoor Research Accelerator Mass Spectrometer Laboratory, and is expected to be the first radiocarbon-dating laboratory in the world dedicated to research rather than to providing a service.
 

Prof. Stephen Weiner’s research is supported by the Exilarch's Foundation; the Maurice and Vivienne Wohl Charitable Foundation; the Estate of Hilda Jacoby-Schaerf; the Ilse Katz Institute for Material Sciences and Magnetic Resonance Research; the Helen and Martin Kimmel Center for Archaeological Science, which he heads; the European Research Council; and the J & R Center for Scientific Research. Prof. Weiner is the incumbent of the Dr. Walter and Dr. Trude Borchardt Professorial Chair in Structural Biology.
 
 
Mobile Lab at the Archaeological Site in Tel es Safi
 
 
 
The Weizmann Institute of Science in Rehovot, Israel, is one of the world's top-ranking multidisciplinary research institutions. Noted for its wide-ranging exploration of the natural and exact sciences, the Institute is home to 2,700 scientists, students, technicians and supporting staff. Institute research efforts include the search for new ways of fighting disease and hunger, examining leading questions in mathematics and computer science, probing the physics of matter and the universe, creating novel materials and developing new strategies for protecting the environment.
 
Weizmann Institute news releases are posted on the World Wide Web at http://wis-wander.weizmann.ac.il/, and are also available at http://www.eurekalert.org/
 
Mobile Lab at the Archaeological Site in Tel es Safi
Scientific Archaeology
English
Yes

New Program To Integrate Archaeology and the Natural Sciences

English

The Weizmann Institute of Science and Bar-Ilan University sign agreement

 

Representatives of Bar-Ilan University and the Weizmann Institute of Science have signed an agreement to collaborate on a unique program for multidisciplinary teaching and research in archaeology and the natural sciences. Modern archaeological research of ancient periods and cultures – in itself not an easy task – can be significantly advanced with the use of scientific approaches and the tools used by researchers in the natural sciences (physics, chemistry, biology, earth sciences and more).

 

The program will allow three scientists who are presently involved in various fields that combine hard science with archaeology (carbon 14 dating, archaeo-botany and geo-archaeology) to carry out research in the framework of the Helen and Martin Kimmel Center for Archaeological Science at the Weizmann Institute, in conjunction with the Martin (Szusz) Department of Land of Israel Studies and Archaeology at Bar-Ilan University. As part of the program, they will teach courses in their areas of scientific archaeology at Bar-Ilan University, and they will also participate in archaeological research there that will incorporate scientific methods and use of the Weizmann Institute research infrastructure. Thus, students in the program will learn from the first to integrate different approaches and research methods from the natural sciences and archaeology. This will ensure they receive truly multidisciplinary training; one that will be expressed in their future research programs.

 

The agreement was signed by Bar-Ilan President Prof. Moshe Kaveh, Rector Prof. Yosef Yeshurun, and Prof. Aren Maeir, Head of the Department of Land of Israel Studies and Archaeology and project Co-director. Weizmann Institute President Prof. Ilan Chet, and Prof. Stephen Weiner, Head of the Kimmel Center for Archaeological Science and project Co-director, signed for the Weizmann Institute.
 
To allow students to become fully versed in both the natural sciences and archaeology, Bar-Ilan University will create several double major programs, starting with students studying for a bachelor’s degree, and later extending to graduate studies.  Each student will take courses in the Department of Land of Israel Studies and Archaeology and one other, such as Geography and Environment (specializing in geo-archaeology), Chemistry (specializing in archaeological chemistry), or the Life Sciences Faculty (specializing in bio-archaeology).
 
 The program will commence in the coming academic year (2006/2007). It will be the first program of its kind in Israel, and one of a very few in the world, and its initiators hope it will make a significant contribution to Israeli archaeology and help put it at the forefront of world-wide research.

 

Prof. Stephen Weiner's research is supported by the Helen and Martin Kimmel Center for Archaeological Science; the Philip M. Klutznick Fund for Research; the Alfried Krupp von Bohlen und Halbach Foundation; and Mr. George Schwartzman, Sarasota, FL.  Prof. Weiner is the incumbent of the Dr. Walter and Dr. Trude Borchardt Professorial Chair in Structural Biology.


Further information on this project can be obtained from the Weizmann Institute Office of Publications and Media Relations, 08-934-3856, or from Bar-Ilan University, 03-531-8121 or 050-540-1410.


The Weizmann Institute of Science in Rehovot, Israel, is one of the world's top-ranking multidisciplinary research institutions. Noted for its wide-ranging exploration of the natural and exact sciences, the Institute is home to 2,500 scientists, students, technicians and supporting staff. Institute research efforts include the search for new ways of fighting disease and hunger, examining leading questions in mathematics and computer science, probing the physics of matter and the universe, creating novel materials and developing new strategies for protecting the environment.

 

Weizmann Institute news releases are posted on the World Wide Web at
http://wis-wander.weizmann.ac.il/, and are also available at http://www.eurekalert.org/.

Scientific Archaeology
English
Yes

Preserved in Crystal

English

A newly discovered source of DNA in fossil bones holds promise for unearthing the past

 
Scientists at the Weizmann Institute of Science recently discovered a new source of well-preserved ancient DNA in fossil bones. Their findings were published in the Proceedings of the National Academy of Sciences (PNAS).
 
Fossil DNA is a potential source of information on the evolution, population dynamics, migrations, diets and diseases of animals and humans. But if it is not well preserved or becomes contaminated by modern DNA, the results are uninterpretable.
 
The scientists, Prof. Steve Weiner and Michal Salamon of the Institute’s Structural Biology Department, working in collaboration with Profs. Baruch Arensburg, Tel Aviv University, and Noreen Tuross, Harvard University, may have found a way to overcome these problems.
 
It was in 1986 that Weiner first reported the existence of crystal clusters in fresh bones. Even when these bones are ground up and treated with sodium hypochlorite – a substance that removes all traces of organic matter – the clusters of crystals remain intact and the organic material embedded in them is unaffected. Now, almost 20 years later, Weiner and Salamon have returned to these findings, reasoning that fossil bones might possess such crystal structures containing preserved ancient DNA.
 
After treating two modern and six fossil animal bones with the sodium hypochlorite, they found that DNA could be extracted from most of these crystal aggregates that is better preserved and contains longer fragments than DNA from untreated ground bone. The technique for reading the DNA worked better, as well, and the use of sodium hypochlorite reduces the possibility of modern contamination.
 
The crystal aggregates act as a "privileged niche in fossil bone," protecting the DNA from hostile environments and leaving it relatively undamaged over time. The team’s findings suggest that the DNA in these aggregates should be preferred, whenever possible, over DNA from untreated bone.
 
This method holds much promise for the future analysis of ancient DNA in bones in yielding more reliable and authentic results than has previously been possible, and may help in unearthing the mysteries of our ancestral past.    
 
Prof. Steve Weiner’s research is supported by the Helen and Martin Kimmel Center for Archaeological Science; the Philip M. Klutznick Fund for Research; the Alfried Krupp von Bohlen und Halbach Foundation; the Women's Health Research Center; and George Schwartzman, Sarasota, FL. Prof. Weiner is the incumbent of the Dr. Walter and Dr. Trude Borchardt Professorial Chair in Structural Biology.
 

The Weizmann Institute of Science in Rehovot, Israel, is one of the world's top-ranking multidisciplinary research institutions. Noted for its wide-ranging exploration of the natural and exact sciences, the Institute is home to 2,500 scientists, students, technicians and supporting staff. Institute research efforts include the search for new ways of fighting disease and hunger, examining leading questions in mathematics and computer science, probing the physics of matter and the universe, creating novel materials and developing new strategies for protecting the environment.


Weizmann Institute news releases are posted on the World Wide Web at
http://wis-wander.weizmann.ac.il/, and are also available at http://www.eurekalert.org/.

Scientific Archaeology
English
Yes

Yaba-Daba-Glue! Stone-Age Use of Collagen Discovered

English
Collagen is one of the latest fads of today's cosmetics industry. Now research at the Weizmann Institute reveals that already back in the stone age, humans were putting this organic compound to use, albeit not in rejuvenating cosmetics. Dr. Arie Nissenbaum discovered an 8,000-year-old cache of collagen used as glue by mysterious Neolithic cavemen who lived in the area of the Dead Sea.
 
"No one knows who these people were and where they came from, but by stone-age standards they surely had mastered at least one type of advanced technology," says Dr. Nissenbaum. Nissenbaum added that the production of collagen glue placed the stone-age cavemen several thousand years ahead of the ancient Egyptians, a surprising technological feat for a group that had not yet mastered pottery.

Nissenbaum studied findings from the Nahal Hemar cave, which was probably used for cult purposes by ancient humans. This cave, located on a cliff near the Dead Sea just northwest of Mt. Sedom, has provided some of the most important regional findings from the late stone age known as the Neolithic period. One of the striking features of the excavated objects is the use of a black substance originally believed to be asphalt. It was applied as a protective lining on rope baskets, containers and embroidered fabrics, as a crisscross-patterned decoration on tops of sculptured skulls and as an adhesive holding together tools and utensils.

When Nissenbaum, who studied asphalt from different archaeological digs, conducted a chemical analysis of the blackish material, he was amazed to discover it was not asphalt at all but rather collagen, the most common fibrous protein in living organisms and a major component of skin, sinews and cartilage. Its chemical composition, as well as an electron microscope analysis of its structure, suggested it was derived from animal skin.

Carbon-14 dating established that the collagen was about 8,100 years old, which coincided with the period when the cave was first used. Although collagen normally quickly converts into gelatin, the Nahal Hemar specimens were exceptionally well preserved due to the region's extremely dry climate.

Natural glues from animal and plant sources were extensively used from antiquity up to the early 20th century. With the Middle East's scarcity of trees, a common source of resin adhesives in ancient Europe, it is hardly surprising that cavemen turned to collagen as a glue. What is surprising, though, is that these people were far ahead of ancient Egyptians who used collagen in its gelatinous form as the basis for the "carpenter's glue" that held together furniture.

While the Egyptians apparently produced their "carpenter's glue" through heating and alkaline solution treatment of animal skins, it's unclear how the Dead Sea cavemen manufactured theirs. It is clear, though, that the stone age craftsmen supplemented the glue with plant-tissue additives, evidently in order to endow it with the appropriate texture.

These findings, reported in the Hebrew- language Archeologia Umada'ei Hateva ("Archaeology and Natural Sciences"), add a new chapter to the history of adhesives and to humankind's technological history in general.
 

Dr. Nissenbaum serves as the Institute's Academic Secretary and conducts research in geochemistry and scientific archaeology. Excavations of the Nahal Hemar Cave were carried out by Prof. Ofer Bar Yosef of the Peabody Museum, Harvard University and David Alon of the Israel Antiquities Authority.

The Weizmann Institute of Science is a major center of scientific research and graduate study located in Rehovot, Israel.

 

Scientific Archaeology
English
Yes

Ancient Ashes Throw New Light on Prehistoric Lifestyles

English
REHOVOT, Israel -- September 24, 1996 -- Prehistoric ashes can now be recognized and analyzed with precision for the first time, thanks to a discovery made by a Weizmann Institute of Science researcher.

The finding, reported in the September issue of the Journal of Archaeological Science, is expected to shed new light on the ways primitive humans used fire, and on their lifestyles and environment. While humans are believed to have first harnessed fire at least 500,000 years ago, ash -- the most direct evidence of fire -- is hard to find and even harder to recognize, because most of the minerals in it are highly reactive and unstable, and begin changing within days of a fire's going out.

As a result, until now evidence of the use of fire has been mostly indirect, coming from blackened bones or fractured flint tools. Now Weizmann Institute's Prof. Steve Weiner -- working with post-doctoral fellow mineralogist Dr. Solveig Schiegl, archaeologist Prof. Ofer Bar-Yosef of Harvard University, and geologist Prof. Paul Goldberg of Boston University -- has traced the chemical transformations that ashes undergo over time, and has discovered that a small group of minerals remains relatively stable and can serve as a telltale sign of ash even after thousands of years.

"We know how to recognize this component now and can look for it, and we have a much better chance of proving something was ash even though it doesn't look like ash at all," says Weiner, a structural biologist who heads the Institute's Environmental Sciences and Energy Research Department.

By analyzing the ashes, scientists can learn about the circumstances in which early humans used fire, which can help explain how they lived, cooked, kept warm, and protected themselves. The ashes and surrounding sediments can also throw light on changes in climate, geology and ecology over the millennia.

"Fire is such an important part of the archaeological record that it may come as a surprise to learn that until now very little analysis has been done on something as basic as ashes," Weiner said.

Archaeologists have assumed that certain sediments are ash if they are found in the form of a hearth, but there has been little scientific analysis of the composition and microscopic structure of ash minerals.

Weiner made his discovery while studying sediments in two northern Israeli caves that had been inhabited from at least 250,000 years ago up to 40,000 years ago.

At the caves, he set up a portable laboratory that included a sophisticated infrared spectrometer, enabling him to analyze mineral samples on-site -- the first time such equipment was used at any archaeological dig. Weiner found that sediments across the floor of one cave, Hayonim, kept giving enigmatic readings on the spectrometer.

The breakthrough came after he realized he had seen the same spectrum in some hearths preserved in the other cave, Kebara.

"That gave me the connection to fire, and I realized that this reading indicated ashes," Weiner said.

To check the finding, Weiner and colleagues conducted experiments in which they burned wood from local trees. They found that the ashes contained a small, relatively stable group of minerals, known as siliceous aggregates, which make up only about 2 percent of the ash's original volume.

It was this fraction that was preserved in the caves. In fact, Weiner found that these siliceous minerals made up a large proportion of the cave sediments, which were up to 3 meters (9 ft.) thick in places -- a finding which suggested the caves had been intensively occupied over millennia.

Weiner and colleagues are now seeking to uncover the record of human habitation in the caves by analyzing the different sediment layers: They hope to distinguish between periods of occupation and non-occupation on the basis that ash would be present only in sediments from periods of occupation.

In addition, by analyzing the other minerals in the ash remnants, they are trying to obtain clues about the types of vegetation present in prehistoric times, which will yield information about the prevailing climate and ecology.

As a result of his discovery, Weiner has recently become the first Western scientist in 60 years to work at China s famous Zhoukoudian cave, home to the bones of Peking Man, believed to be among the first humans to use fire. Weiner will spend the next year analyzing samples he brought back from China in May, and trying to determine precisely what they comprise, whether there were indeed fires at the site, and whether they were lit by humans.

Prof. Stephen Weiner holds the Weizmann Institute's I.W. Abel Chair of Structural Biology and heads the Sussman Family Center for the Study of Environmental Sciences.

The Weizmann Institute of Science is a major center of scientific research and graduate study located in Rehovot, Israel.
Scientific Archaeology
English
Yes

Prehistoric Fires Spark a Scientific Leap Forward

English
By Miriam Bulwar David-Hay
 
REHOVOT, Israel -- Somewhere, back in the mists of time, a primitive human realized how to light a fire. Suddenly, people could cook their food, keep warm in the cold, scare off predators, and, perhaps not least of all, gather around a central meeting place to enjoy one another s company after a hard day.

Cut to 1996. Those fires, and the people who sat around them, are long dead. The only memories of them remain in crumbling bones and tools and piles of dirt; our ancestors and all the complex doings of their lives have, as the saying goes, returned to dust and ashes.

And yet, for some people, the dust and ashes still breathe. Prof. Steve Weiner, a structural biologist at Israel's Weizmann Institute of Science, is one such person. In fact, he has spent the past decade studying dust and ashes -- and in the process has made a discovery that could move knowledge of prehistoric humanity several steps forward.

Weiner has discovered how to identify and analyze ancient ashes -- the most direct evidence of fire -- after they have almost entirely disintegrated. It may seem astonishing that, with science as advanced as it is, no-one could do so until now.

And yet this was the case: Ash is largely made up of unstable minerals that react and change constantly and are difficult to recognize even days after a fire goes out. Archaeologists have assumed certain sediments are ash, but until now there has been little scientific analysis of the composition of ash.

"What we found is that while most ash minerals do change over time, a very small component -- only about 2 percent of the original volume of the ash -- stays relatively stable," Weiner says."We know how to recognize this component now and can look for it, and we have a much better chance of proving something was ash even though it might not look like ash at all."

The discovery was made while Weiner was working in two prehistoric caves in northern Israel with postdoctoral fellow Dr. Solveig Schiegl, archaeologist Dr. Ofer Bar-Yosef of Harvard University, and geologist Dr. Paul Goldberg of Boston University. The finding is reported in the September issue of the Journal of Archaeological Science, and has already earned the 48-year-old South African-born Weiner a rare honor: He is the first Western scientist in 60 years invited to work at China's famous Zhoukoudian cave, home to the bones of Peking Man, believed to be among the first humans to use fire deliberately, some 500,000 years ago.

Weiner, who heads the Weizmann Institute s Environmental Sciences and Energy Research Department, admits he made the discovery almost serendipitously. He had chosen the Israeli caves -- which had been inhabited as far back as 250,000 years ago -- to study their rich collection of bones and artifacts.

He took with him a portable infrared spectrometer, a device that enabled him to analyze mineral samples on-site -- the first time this equipment, common in chemistry laboratories, was used at an archaeological dig. The device helped reveal that animal bones found piled in one cave, Kebara, were placed that way by the cave dwellers and were not a result of dissolution in some parts of the cave and preservation in others.

This led to the conclusion that Neanderthals were trend-setters in home design: they were among the first to divide their caves into separate areas of use, with a hearth at one end and a garbage dump at the other.

But when Weiner used the equipment in a second cave, Hayonim, he ran into a puzzle." I kept getting enigmatic readings on the spectrometer from all over the cave floor; I couldn t identify the major component of the sediments," he says. "It was only after some time that I realized I had gotten the same reading from some of the hearths in Kebara. That gave me the connection to fire, and I understood that this reading indicated ashes. Even though there were almost no hearths in Hayonim, there was ash all over the floor."

To test his theory, Weiner and his colleagues collected wood from local trees, burned it, and studied the results.

They found that a very small proportion of the ash was composed of a relatively stable group of ash minerals, known as siliceous aggregates. It was these siliceous minerals that made up a large proportion of the sediments -- in places up to three meters (9 ft.) thick -- on the floor of Hayonim and in the hearths of Kebara.The abundance of these minerals indicates that humans inhabited the caves intensively over periods of thousands of years.

"These caves were probably coveted real estate that were inhabited over a long period, and there is a lot to be learned here," Weiner says.

He and colleagues are now seeking to uncover the record of human habitation in the area by studying the sediment layers: They assume that ash would be present only in sediments from periods of habitation.In addition, the ashes and their surrounding sediments may throw light on changes in ecology, climate, and geology over the millennia.

At the same time, Weiner is busy analyzing samples he brought back from China this past May. He cannot yet say exactly what is in them: Unlike the well-preserved Israeli caves, Zhoukoudian has been cut open and exposed to the elements for years, and the sediments are diluted.

"At present scientists agree that Zhoukoudian was the earliest proven use of fire by man, based on evidence such as burnt bones and what was assumed to be ash," Weiner says. "I can t confirm or deny that yet; we re still analyzing samples."

Weiner is working on both the Chinese and the Israeli material with Drs. Bar-Yosef and Goldberg. They plan to return to China next May to try to complete the work. "Earlier investigators assumed certain things, based mainly on what they saw with the naked eye," Weiner says. "But we need to re-examine everything. We need to know exactly what the Zhoukoudian sediments are made of, and, if there proves to be ash, that it didn t come from a brush fire, but from one that was man-made and deliberate."

Meanwhile, applying science to archaeology has already shed new light on age-old secrets.

Prof. Weiner holds the I.W. Abel Chair of Structural Biology and heads the Sussman Family Center for the Study of Environmental Sciences at the Weizmann Institute of Science.

 

The Weizmann Institute of Science is a major center of scientific research and graduate study located in Rehovot, Israel.
Scientific Archaeology
English
Yes

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