Life-Giving Research

English

Hannah Esther Angel Kaman. Sucessful pregnancy

 
 
 

 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Basic research often takes years to be translated into medical applications, but once in a while, a finding can change lives almost immediately.

In 2006, Prof. Nava Dekel of the Biological Regulation Department, together with doctors in the IVF unit of Kaplan Medical Center, made the surprising discovery that performing a uterine biopsy – causing a slight injury to the lining of the uterus – just before a woman undergoes in vitro fertilization (IVF) doubles the chances of a successful pregnancy. The injury apparently provokes a response that makes the uterus more receptive to the embryo's implantation.

The next year, Dekel was in Toronto, Canada, giving a lecture in the framework of the Weizmann Women and Science series, organized by Weizmann Canada. That lecture was reported in a local Jewish newspaper, where it caught the attention of Howard and Roslyn Kaman. After years of unsuccessful fertility treatments, failed IVF and miscarriages, the couple gained new hope. They contacted Dekel, and she referred them to Drs. Amichai Barash and Irit Granot, who had participated in the original research along with Drs. Yael Kalma and Yulia Gnainsky of the Weizmann Institute.

The Rehovot doctors provided a detailed description of the procedure, which was then performed in a fertility clinic in Toronto. The result: A healthy baby girl, Hannah Esther Angel Kaman, was born this past October.   

Prof. Nava Dekel's research is supported by the Dwek Family Biomedical Research Fund; the Kirk Center for Childhood Cancer and Immunological Disorders; and the Dr. Pearl H. Levine Foundation for Research in the Neurosciences. Prof. Dekel is the incumbent of the Philip M. Klutznick Professorial Chair of Developmental Biology.
 
Hannah Esther Angel Kaman. Sucessful pregnancy
Life Sciences
English

The Secrets of Hybrid Vigor

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Yeast gene correlations reveal hybrid vigor secret

 

 

 
 
Hybrids fascinated Charles Darwin, supplying him with intriguing clues into the evolution of species. Now Weizmann Institute scientists are using hybrid organisms to investigate the genetic machinery of evolution.
 
Prof. Naama Barkai of the Molecular Genetics Department, Prof. Avraham Levy of the Plant Sciences Department, and research students Itay Tirosh and Sharon Reikhav focused on differences in gene expression between species.
 
In a study published in Science, the researchers found that hybrid yeast fit Darwin’s description of organisms that gain in crossbreeding – they grew faster than either parent yeast. One theory for hybrid vigor is based on the fact that two types of DNA sequences affecting gene expression can complement each other: sequences called “cis,” which are physically linked to the gene, and those called “trans,” located elsewhere in the genome. In certain crosses, a strong cis in a gene inherited from one parent might be combined with the strong trans of the gene inherited from the other parent, leading to extra gene expression. And that’s exactly what the researchers found: an especially high level of expression for certain genes.
 
The mechanisms for novel patterns of gene expression in hybrids may explain why they can be phenotypically different from either parent. Although hybrids are mostly sterile, several species do not rely solely on sexual reproduction, and genome hybridity is one way to rapidly gain new traits. Levy: “We are interested in applying the lessons from yeast to bread wheat, a species that contains different genomes merged into the same nucleus.”
 
Prof. Naama Barkai’s research is supported by the Kahn Foundation; the Helen and Martin Kimmel Award for Innovative Investigation; the Carolito Stiftung; the Minna James Heineman Stiftung; the PW-Iris Foundation; and the PW-Jani. M Research Fund.
 
Prof. Avraham Levy is the incumbent of the Gilbert de Botton Professorial Chair of Plant Sciences.
Correlations between various “cis” and “trans” effects on gene expression, under different conditions. The correlations range from high (red) to low (blue)
Life Sciences
English

Cells Move like Millipedes

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 A scanning electron microscope image of a white blood cell with “legs”

 

 

 
 
How do white blood cells – immune system “soldiers” – cross the blood vessel barrier to get to the site of infection or injury? Until recently, it was believed that these cells advanced like inchworms, sticking front and back, folding and extending to push themselves forward. Yet in research recently published in Immunity, Prof. Ronen Alon and his research student, Ziv Shulman, of the Immunology Department showed that the rapid movement of the white blood cells is more like that of millipedes.
 
The cell creates numerous tiny “legs” no more than a micron in length, rich in adhesion points that bind to partner adhesion molecules on the surface of the blood vessels. Tens of these legs attach and detach in sequence within seconds, enabling them to move rapidly while keeping a good grip on the vessels’ sides.
 
Images produced by scanning and transmission electron microscopes, taken by Drs. Eugenia Klein and Vera Shinder of the Institute’s Electron Microscopy Unit, showed that upon attaching to the blood vessel wall, the white blood cell legs “dig” themselves into the endothelium, pressing down on its surface. The scientists believe that the tiny legs are trifunctional: used for gripping, moving and sensing distress signals from the damaged tissue.
 
In future studies, the scientists plan to check whether aggressive immune reactions (such as in autoimmune diseases) can be regulated by interrupting the digging of immune cell legs into the endothelium. They also plan to investigate whether cancerous blood cells metastasize through the blood stream using similar mechanisms.
 
Prof. Ronen Alon’s research is supported by the De Benedetti Foundation-Cherasco 1547. Prof. Alon is the incumbent of the Linda Jacobs Chair in Immune and Stem Cell Research.
 
 
A scanning electron microscope image of a white blood cell with “legs”
Life Sciences
English

Built to Fold

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Embedded in the genome is yet another code made up of two-letter signals that repeat themselves periodically.
 
Discovered in 2006 by Dr. Eran Segal of the Computer Science and Applied Mathematics Department and his group, this code appears at intervals of 10 base pairs (the “letters” of the genetic code) in certain DNA sequences. It facilitates the bending of segments of about 150 base pairs into protein-DNA complexes called nucleosomes – neat, spherical beads strung on the DNA strand.
 
Two new studies by his group, which appeared recently in Nature and Nature Genetics, provide evidence that this code is universal, from yeast to humans, and that it helps to shape those organisms and streamline the process of gene expression. The group, including research students Noam Kaplan and Yair Field, along with Yaniv Lubling, carried out this research in collaboration with the groups of Jonathan Widom at Northwestern University in Evanston, Illinois, Jason Lieb at the University of North Carolina in Chapel Hill, North Carolina, and Tim Hughes at the University of Toronto. “In the 2006 study, we worked with several hundred DNA sequences. Today, with new advances in technology, we can test something like 10 million,” says Segal.
 
In the second study, Segal and his team revisited previous Institute research from the group of Prof. Naama Barkai of the Molecular Genetics Department. They discovered that differences in gene expression are tied to changes in the placement of nucleosomes, which, in turn, might constitute a previously unidentified genetic mechanism for achieving change across evolution.
 
Dr. Eran Segal’s research is supported by the Chais Family Fellows Program for New Scientists; the Hana and Julius Rosen Fund; and the Cecil and Hilda Lewis Charitable Trust.
Life Sciences
English

Clinging for Dear Life

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Xeroderma pigmentosum is a rare hereditary disease in which sun-damaged skin is unable to mend itself. Its sufferers run a risk of skin cancer that is 5,000 times higher than normal.
 
Ten years ago, scientists discovered that the genetic defect behind one form of this disease, known as xeroderma pigmentosum variant (XPV), is a mutant version of an enzyme called DNA polymerase eta, which allows the cell to tolerate sun-induced DNA damage without removing this damage from the DNA molecule. In new research published in the Proceedings of the National Academy of Sciences (PNAS), USA, Prof. Zvi Livneh and research student Omer Ziv of the Biological Chemistry Department reveal how XPV cells manage to survive despite having this mutated enzyme – and at what cost.
 
With help from Nicholas Geacintov of New York University, and Satoshi Nakajima and Akira Yasui of Tohoku University, Japan, the researchers found that at least three other repair enzymes fill in when the mutated version cannot function. Yet even this combined effort of the three substitutes – DNA polymerases iota, kappa and zeta – only manages to do part of the job, increasing the risk of DNA errors 10- to 20-fold. “What’s fascinating about these findings,” says Livneh, “is that they represent an extreme example of the biological drive to preserve life, even when the price is a heavy load of genetic mutation and a high risk of cancer.”
 
Prof. Zvi Livneh’s research is supported by the Helen and Martin Kimmel Institute for Stem Cell Research; the estate of Lore F. Leder; and Esther Smidof, Switzerland. Prof. Livneh is the incumbent of the Maxwell Ellis Professorial Chair in Biomedical Research.
Life Sciences
English

The Witches of Galilee

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Ancient burial suggest shamanism
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Fifty tortoise shells, a human foot and the body parts of creatures that include a wild boar, an eagle and a leopard – all found in the 12,000-year-old grave of a petite elderly woman in the Hilazon Tachtit Cave – suggest that this ancient resident of Israel's western Galilee was one of the world's earliest shamans. She lived in a time of dramatic transitions; the Natufians, to whom she belonged, formed the first society known to have abandoned a nomadic lifestyle. Settling down entailed yet another major shift: from hunting and gathering to farming.

Such sweeping transitions create unprecedented social and spiritual needs, says team leader Dr. Leore Grosman of the Hebrew University of Jerusalem's Institute of Archaeology, who conducted the study with Dr. Natalie Munro of the University of Connecticut and Jerusalem colleague Dr. Anna Belfer-Cohen. Shamans serve as healers, messengers and magicians while mediating between the human and spiritual worlds. Though they have been common to cultures around the world, including many existing today, no burial site of theirs has ever before been found in the Mediterranean area.

Dr. Grosman is now a postdoctoral fellow in the lab of Prof. Uzy Smilansky of the Weizmann Institute's Physics of Complex Systems Department, who has developed a method for characterizing ancient artifacts based on their three-dimensional images. Grosman is applying this method to addressing various issues in prehistoric archaeology, such as sorting and classifying stone tools or determining whether a particular object had originated at the site of the excavation or had been brought there from a distance, for example, by river flow.  

Prof. Uzy Smilansky's research is supported by the Minerva Center for Nonlinear Physics of Complex Systems. Prof. Smilansky is the incumbent of the Professor Wolfgang Gentner Professorial Chair of Nuclear Physics.

Excavation site of the Natufian cemetery in a cave overlooking Nahal Hilazon in western Galilee
Scientific Archaeology
English
Yes

Closing the Gap

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 Profs. Daniel Zajfman, Elizabeth Blackburn, Adi Kimchi and Dr. Maya Bar Sadan. Women in science

 

 

 

 

 
 
 
 
 
 
 
 
 
 
 
 
Dr. Maya Bar Sadan (in the photo, right) is one of 11 young women scientists who, after completing their Ph.D. studies with honors at an Israeli university or other academic institution, each recently received an award of about $20,000 a year, for two years. These awards, which will help them pursue postdoctoral studies at leading universities abroad, have been granted within the framework of the Weizmann Institute's National Postdoctoral Award Program for Advancing Women in Science, now in its second year. It is aimed at assisting highly talented young women to work toward a career in the sciences: natural (physics, chemistry and the life sciences) or exact (mathematics and computer science). The goal of the program is to begin closing the gap between the numbers of male and female scientists in the highest ranks of academia.

Five of this year's recipients conducted their doctoral studies at the Weizmann Institute of Science, two at the Hebrew University of Jerusalem, two at Tel Aviv University, one at the Technion – Israel Institute of Technology and one at Ben-Gurion University of the Negev.

Recipients of the awards were selected by a special Feinberg Graduate School committee, headed by the Weizmann Institute  President's Adviser for Advancing Women in Science, Prof. Adi Kimchi.  
 
The program is supported by the Clore Foundation – Sara Lee Schupf Postdoctoral Awards; and Mike and Valeria Rosenbloom through the Mike Rosenbloom Foundation.  
 
 
Profs. Daniel Zajfman, Elizabeth Blackburn, Adi Kimchi and Dr. Maya Bar Sadan. Women in science
English

Vive la Difference

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chemotherapy alters protein levels in cancer cells
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
What's the difference between cancer cells that are killed by chemotherapy and the ones that survive the treatment? To tackle this question, Ariel Cohen together with Naama Geva-Zatorsky and Eran Eden in the lab of Prof. Uri Alon of the Molecular Cell Biology Department developed an original method for imaging thousands of living cells and analyzing their activities automatically by computer.
 
The team members worked for several years to complete the project, which entailed observing the behavior of over 1,000 different proteins, tagging specific proteins in each group of cancer cells and each time capturing a series of time-lapsed images over 72 hours. A chemotherapy drug was introduced 24 hours into this period, after which the cells began the process of either dying or defending themselves against the drug.
 
The team's efforts have produced a comprehensive library of tagged cells, images and data on cancer cell proteins – a virtual gold-mine for further cancer research. And they succeeded in identifying two proteins that seem to play a role in cancer cell survival. One of them, known by the letters DDX5, is a multi-tasking protein that, among other things, plays a role in initiating the production of other proteins. The other, RFC1, also plays a variety of roles, including directing the repair of damaged DNA. When the researchers blocked the production of these proteins in the cancer cells, the drug became much more efficient at wiping out the growth. Says Cohen: "We were able to pinpoint possible new drug targets and to see how certain activities might boost the effectiveness of current drugs."  
 
Prof. Uri Alon's research is supported by the Kahn Foundation; Keren Isra - Pa'amei Tikva; and the Minerva Junior Research Group on Biological Computation.
 
 
 
chemotherapy alters protein levels in cancer cells
Life Sciences
English

Part and Particle

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quantum Hall device in which even fractional charges were measured
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 

 
 
When is dividing by four more complicated than dividing by three or five? When the thing divided is the charge of an electron. A decade after they discovered “quasiparticles” with charges of one-third and one-fifth, Weizmann Institute physicists have succeeded in demonstrating, for the first time, the existence of quasi-particles with one-quarter the charge of an electron. This finding could be a first step toward creating exotic types of quantum computers that might be powerful, yet highly stable.
 
Fractional electron charges were first predicted over 20 years ago, and were found by the Weizmann group some ten years ago. Although electrons are indivisible, if they are confined to a two-dimensional layer inside a semiconductor, chilled down to a fraction of a degree above absolute zero and exposed to a strong magnetic field that is perpendicular to the layer, they effectively behave as independent particles, called quasiparticles, with charges smaller than that of an electron.
 
The experiment done by research student Merav Dolev in Prof. Moty Heiblum’s group, in collaboration with Drs. Vladimir Umansky and Diana Mahalu and Prof. Adi Stern, all of the Condensed Matter Physics Department, owes the finding of quarter-charge quasiparticles to an extremely precise setup and unique material properties: The gallium arsenide material they produced for the semiconductor was among the purest in the world.  
 
Prof. Moty Heiblum’s research is supported by the Joseph H. and Belle R. Braun Center for Submicron Research. Prof. Heiblum is the incumbent of the Alex and Ida Sussman Professorial Chair of Submicron Electronics.
 
An artist’s impression of the quantum Hall device in which even fractional charges were measured
Space & Physics
English

Large-Scale Invasion

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 mimivirus with a star-shaped opening
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Weizmann Institute scientists have revealed certain mechanisms by which a mimivirus – a virus so called because it was originally thought to mimic bacteria in various aspects of their behavior – invades amoeba cells.
 
The mimivirus, known, among other things, for its exceptional size – it is five to ten times larger than any other known virus – was discovered only in the late 20th century, as its extraordinary size made it impossible to identify it by regular means. It contains much more genetic material than regular viruses, a feature that forces the mimivirus to develop particularly efficient methods for introducing its viral DNA into the host cell and for inserting its genetic “parcel” into a protein “container” during the production of new viruses in the host cell.

Prof. Abraham Minsky and graduate students Nathan Zauberman and Yael Mutsafi of the Organic Chemistry Department, together with Drs. Eugenia Klein and Eyal Shimoni of Chemical Research Support, have now discovered the details of some of the methods used by this virus. The scientists have obtained, for the first time, three-dimensional pictures of the openings through which the viral genetic material is injected into the infected cell, and of the process by which this genetic material is inserted into the protein container.

The study of the mimivirus’s life cycle, from cellular infection to the production of new viruses, may yield valuable insights into the mechanisms of action of numerous other viruses, including those that cause human diseases. Such insights could enable scientists to interrupt the infection cycle, blocking viral diseases.

Prof. Abraham Minsky’s research is supported by the Helen and Milton A. Kimmelman Center for Biomolecular Structure and Assembly; the Irving and Cherna Moskowitz Center for Nano and Bio-Nano Imaging; and the Wolfson Family Charitable Trust. Prof. Minsky is the incumbent of the Professor T. Reichstein Professorial Chair.
 
DNA invades the host cell through a star-shaped opening
Chemistry
English

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